"WHO" says Sperm DNA Fragmentation test should be part of the semen analysis patient profile to obtain a comprehensive assessment
Semen Analysis PackageAutomated Semen Analysis + DNA Fragmentation Test
You pay₹6,000₹7,500
Healthy DNA: two unbroken strands
Semen analysis key highlights
A semen analysis you can see for yourself
Fully automated analyser
Your semen analysis is performed on a fully automated analyser, which measures every sample the same way.
Real images on your report
Your report shows actual microscope images of your own sample, not just numbers.
Sperm vitality included
We check how many of your sperm are alive as part of the semen analysis. Many labs charge for this test separately.
Watch your sperm on video
Scan the QR code on your report to watch the video of your sperm analysis.
Inside the sperm
01 · Head
The head carries the DNA
Its nucleus holds 23 chromosomes, tightly packed: half of your baby's genes. The cap over its tip, the acrosome, holds enzymes that help it through the egg's outer layer.
Checked in your package:
- DNA fragmentation
- Morphology
02 · Neck
The neck links head and tail
A short, flexible joint. It carries the centriole, which helps a fertilised egg organise its first cell division.
Checked in your package:
- Morphology
03 · Midpiece
The midpiece powers the swim
Mitochondria wound round it in a spiral make the energy the tail needs to beat.
Checked in your package:
- Motility
- Morphology
04 · Tail
The tail drives it forward
It beats in a whip-like wave that pushes the sperm ahead. Your semen analysis measures how many sperm move, and how well: their motility.
Checked in your package:
- Motility
- Morphology
Illustration, not to scale
The sperm's journey
01 · Vagina
Millions set off
One ejaculate carries millions of sperm. The vagina is acidic, which is hard on sperm, and semen shields them from it only for a while.
02 · Cervix
The cervix screens them
Its mucus filters out sperm that swim poorly or are badly shaped. Around ovulation it turns thin and slippery, and lets the strong swimmers through.
03 · Uterus
Up through the uterus
Those that get through swim on toward the two fallopian tubes, helped along by contractions of the uterus. Usually only one tube holds an egg.
04 · Fallopian tube
A few thousand reach the tubes
There they are held in the lining of the tube, where sperm can survive for up to 7 days. Near ovulation they are released, ready to fertilise and swimming harder.
05 · The egg
A few hundred reach the egg
Guided toward it, they push through the cells around the egg to its outer shell, the zona pellucida.
06 · Fertilisation
Only one gets in
The first sperm to fuse with the egg sets off a change that hardens its shell, so no other can enter. Its DNA joins the egg's: half of your baby's genes. So the DNA it carries matters.
What is a DNA fragmentation test?
01 · Your DNA
Your sperm carry your DNA
That is important genetic information: half of your baby's genes, coiled tight in the head of every sperm. Don't you think you should know whether your sperm carry healthy DNA or not?
02 · The blind spot
A sperm can look perfect and still carry broken DNA
Count, movement and shape don't show what's inside. The WHO semen manual notes that DNA damage is only partly related to semen quality.
03 · The damage
Breaks build up quietly
Heat, smoking, infection, a varicocele and age are among the causes linked to sperm DNA damage.
04 · The measure
A DNA fragmentation test puts a number on it
It finds the share of your sperm with broken DNA: your DNA Fragmentation Index (DFI). On our report, below 15% is normal and above 30% is high.
- Below 15%: Normal
- 15–30%: Moderate
- Above 30%: High
Under the microscope
A semen analysis examines sperm.A DFI test examines their DNA.
Move the lens over the slide. In the sperm chromatin dispersion (SCD) test we use, sperm with intact DNA form a halo; sperm with fragmented DNA form a small halo or none.
Why it matters
High DNA fragmentation is linked to
How the embryo develops
The WHO semen manual notes that DNA damage may affect embryo development, implantation and pregnancy.
Failed IUI or IVF
European urology guidelines link high DNA damage to lower pregnancy rates after IUI and poorer IVF results.
Repeated miscarriages
High DNA fragmentation is associated with recurrent pregnancy loss, so guidelines advise testing the male partner.
Unexplained infertility
It has been linked to infertility in men whose semen report is normal.
Your result
How your DFI is read
Drag the slider to see a result. The sperm around you change with it.
Most sperm carry intact DNA.
Ranges as printed on our report. Your doctor reads your DFI with the rest of your results.
Why a package
Why we put both tests in one package
A semen analysis and a DNA fragmentation test look at different things. Done together, on one sample, they give a complete assessment of your sperm.
Automated semen analysis
Report in 4 hrs
What it looks at
How many sperm there are, how they move, their shape and how many are alive.
- Count, motility, morphology, vitality
- Volume, pH, viscosity
- Pus cells / RBCs
- Microscope images
- Sperm video by QR code
Sperm DNA fragmentation
Report in 5 days
What it looks at
The DNA inside them: how many of your sperm carry broken DNA.
- DNA Fragmentation Index (SCD method)
- 5-level halo classification
- Annotated microscope images of the DNA analysis
A normal semen report can hide DNA damage
A semen analysis looks at sperm, not inside them. Fertility guidelines note that a sperm that looks and moves normally can still carry a high level of DNA fragmentation.
WHO lists the DNA test beyond the basics
The WHO semen manual describes sperm DNA fragmentation testing in its extended examination, a step beyond the basic semen analysis.
One sample, one visit
Both tests run on the same sample, so you come in once and get two reports. The semen analysis comes free.
The evidence
What WHO and fertility guidelines say
A semen report alone can't predict fertility
“For an individual patient, a semen analysis is never prognostic of fertility…”
DNA testing adds what a semen report misses
“Since [sperm DNA fragmentation] is only partially related to semen quality, it could represent an important addition in the work-up of male infertility…”
DNA damage can affect the embryo and the pregnancy
“…multiple meta-analyses published in recent years indicate that [sperm DNA fragmentation] may affect embryo development, implantation, and pregnancies in both natural and assisted reproduction.”
European Association of Urology
Guidelines on Sexual and Reproductive Health, 2026
Strong recommendation
“Perform sperm DNA fragmentation (SDF) testing in the assessment of couples with recurrent pregnancy loss from natural conception and failure of ART or males with unexplained infertility.”
American Urological Association & ASRM
Diagnosis and Treatment of Infertility in Men, 2020 (amended 2024)
“Even with a ‘normal’ [semen analysis], a sperm that appears morphologically and functionally normal may … have a high level of DNA fragmentation.”
Is it for you?
Who should take the DNA test
Guidelines recommend it after
- Two or more miscarriages
- A failed IUI or IVF cycle
- Infertility with no clear cause
European Association of Urology, 2026: strong recommendation.
Also worth asking about if you have
- A normal semen report, but no pregnancy yet
- Varicocele (swollen veins in the scrotum)
- Smoking, obesity or regular heat exposure
- Aged 40 or over
- Past genital infection or chemotherapy
Book now
Book the Semen Analysis Package
One sample, collected in a private room at our centre. Your semen analysis report reaches you in 4 hrs, and your DNA fragmentation report in 5 days, both on WhatsApp.
- Fully confidential
- NABL accredited
- DNA report in 5 days
Semen Analysis Package
Semen Analysis + DNA Fragmentation
₹6,000₹7,500
- Automated semen analysis, with vitality
- Microscope images and a QR code to your sperm video
- DNA Fragmentation Index (SCD method)
- 5-level halo classification
- Annotated microscope images of the DNA analysis
- Visual charts against reference ranges
- Doctor-ready for IUI / IVF / ICSI planning
- Semen analysis report in 4 hrs, DNA report in 5 days, on WhatsApp
Not sure the package is for you?
Leave your number and tell us what your doctor has advised. We'll call you back to talk it through, in confidence.
- Callback within 15 minutes
- Fully confidential
- No charge for advice
Get a Free Callback
Leave your number and we'll call you back within 15 minutes to confirm.
Frequently Asked Questions
Quick answers about the semen analysis package, the DNA fragmentation test, preparing for it and your reports.
How your semen analysis
package works
From booking to report, in four simple steps.
Book or Ask for a Callback
Book the test online, or leave your number and we will call you back with a slot.
Visit Your Simira Centre
Come in after 2–7 days of abstinence. Your sample is collected in a private, comfortable room.
Tested in Our Lab
Your sample goes through our automated semen analyser, and its DNA is tested with the SCD method.
Two Reports on WhatsApp
Your semen analysis report reaches you in 4 hrs; the DNA fragmentation report in 5 days.